Coding

Part:BBa_K4343102

Designed by: Zheng Zhe   Group: iGEM22_NNU-China   (2022-10-09)


BgElo9

BgElo9 encodes ∆-9 elongase from Blattella germanica which functions in the Δ-9 elongase/desaturase pathway and could convert C18:2 to C20:2.

Characterization

Heterologous expression of BgElo9 could enable engineered Y. lipolytica to produce C20:2 fatty acids that couldn’t be produced in wild-type Y. lipolytica.

Literature

Some studies have employed C16/18 elongase from Blattella germanica to produce high yield omega-3 fatty acids.

Reference

Juárez, M. P., 2004. Fatty acyl-CoA elongation in Blatella germanica integumental microsomes. Archives of Insect Biochemistry and Physiology. 56, 170-178.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 804
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI site found at 528
    Illegal BsaI.rc site found at 57
    Illegal BsaI.rc site found at 1004


[edit]
Categories
Parameters
None